pan hdac inhibitor sb939  (Santa Cruz Biotechnology)

Journal: Cells

Article Title: Functional Characterization of LTR12C as Regulators of Germ-Cell-Associated TA-p63 in U87-MG and T98-G In Vitro Models.

doi: 10.3390/cells14110852

Figure Lengend Snippet: Figure 2. Dual DNMT1 and HDACs inhibition induced the expression of LTR12C-driven genes in the GBM cell models. (A) Overlap between the genes upregulated by cotreatment with DAC and SB939

Article Snippet: The hypomethylating agent 5-aza-2′-deoxycitidine (DAC, Selleckchem, S1200, Houston, TX, USA) was resuspended to a final concentration of 100 mM in Dimethyl sulfoxide (DMSO); the pan-HDAC inhibitor SB939 (Selleckchem, S1515, Houston, TX, USA) was resuspended in DMSO to reach a final concentration of 50 mM and subsequently used to treat the cells.

Techniques: Inhibition, Expressing

Journal: Cells

Article Title: Functional Characterization of LTR12C as Regulators of Germ-Cell-Associated TA-p63 in U87-MG and T98-G In Vitro Models.

doi: 10.3390/cells14110852

Figure Lengend Snippet: Figure 4. DNMT1 and HDACs combined inhibition affected the expression of the cell cycle-related genes. (A) Quantitative RT-PCR of the CDKN1A (p21) mRNA levels after the treatment with 500 nM DAC and/or 500 nM SB939 in the U87-MG (up) and T98-G (down). (B) Quantitative RT-PCR of the CDK4 mRNA levels after treatment with 500 nM DAC and/or 500 nM SB939 in the U87-MG (up) and T98-G (down). (C) Quantitative RT-PCR of the CDK6 mRNA levels after the treatment with 500 nM DAC and/or 500 nM SB939 in the U87-MG (up) and T98-G (down). The results are expressed as 2−∆∆Ct. (D) Representative Western blot and densitometric analysis of CDKN1A (p21) protein levels in the U87-MG (left) and T98-G (right) cells after the inhibition of DNMT1 and/or HDACs. The data are expressed as the mean ± SD, * p value ≤0.05; ** p value ≤0.01; *** p value ≤0.001; test—unpaired two tailed t-test of treated samples vs. vehicle, corrected for multiple testing with the Bonferroni method.

Article Snippet: The hypomethylating agent 5-aza-2′-deoxycitidine (DAC, Selleckchem, S1200, Houston, TX, USA) was resuspended to a final concentration of 100 mM in Dimethyl sulfoxide (DMSO); the pan-HDAC inhibitor SB939 (Selleckchem, S1515, Houston, TX, USA) was resuspended in DMSO to reach a final concentration of 50 mM and subsequently used to treat the cells.

Techniques: Inhibition, Expressing, Quantitative RT-PCR, Western Blot, Two Tailed Test

Journal: Cells

Article Title: Functional Characterization of LTR12C as Regulators of Germ-Cell-Associated TA-p63 in U87-MG and T98-G In Vitro Models.

doi: 10.3390/cells14110852

Figure Lengend Snippet: Figure 5. DNMT1 and HDAC combinatory inhibition induced the expression of pro-apoptotic genes. (A,B) Quantitative RT-PCR of BBC3 (PUMA) and PMAIP (NOXA) mRNA expression levels after the treatment with 500 nM DAC and/or 500 nM SB939 in the U87-MG (A) and T98-G (B) cells. The results are expressed as 2−∆∆Ct. (C) Representative Western blot and densitometric analysis of the PARP1 protein cleavage after the treatment with 500 nM DAC and/or 500 nM SB939 in the U87-MG (left) and T98-G (right) cells. (D) Representative Western blot analysis of the p53 protein expression level after the treatment with 500 nM DAC and/or 500 nM SB939 in the T98-G cells (left) and densitometric analysis (right). The data is expressed as the mean ± SD, * p value ≤0.05; ** p value ≤0.01; *** p value ≤0.001; test—unpaired two tailed t-test of treated samples vs. vehicle, corrected for multiple testing with the Bonferroni method.

Article Snippet: The hypomethylating agent 5-aza-2′-deoxycitidine (DAC, Selleckchem, S1200, Houston, TX, USA) was resuspended to a final concentration of 100 mM in Dimethyl sulfoxide (DMSO); the pan-HDAC inhibitor SB939 (Selleckchem, S1515, Houston, TX, USA) was resuspended in DMSO to reach a final concentration of 50 mM and subsequently used to treat the cells.

Techniques: Inhibition, Expressing, Quantitative RT-PCR, Western Blot, Two Tailed Test

Journal: Cells

Article Title: Functional Characterization of LTR12C as Regulators of Germ-Cell-Associated TA-p63 in U87-MG and T98-G In Vitro Models.

doi: 10.3390/cells14110852

Figure Lengend Snippet: Figure 6. Dual inhibition of DNMT1 and HDACs reduced the cell viability in the two glioblastoma cell models. (A) MTS analysis of the U87-MG (left) and T98-G (right) cells treated with 500 nM DAC and/or 500 nM SB939 for 96 h. (B) Dose–response curves of DAC, SB939, and DAC and SB939 in the U87-MG (left) and T98-G (right) cells treated for 96 h. The percentage of cell prolif- eration was calculated from Incucyte Live-Cell Analysis data as the percentage of the ratio of cell number of treated samples over the cell number of the control group. (C) Incucyte® Live-Cell Analysis

Article Snippet: The hypomethylating agent 5-aza-2′-deoxycitidine (DAC, Selleckchem, S1200, Houston, TX, USA) was resuspended to a final concentration of 100 mM in Dimethyl sulfoxide (DMSO); the pan-HDAC inhibitor SB939 (Selleckchem, S1515, Houston, TX, USA) was resuspended in DMSO to reach a final concentration of 50 mM and subsequently used to treat the cells.

Techniques: Inhibition, Cell Analysis, Control